Fig. 6: Integrated analysis of transcriptome and translatome identifies that cell cycle pathway gene MCM5 is a target of FIBCD1. | Cell Death & Disease

Fig. 6: Integrated analysis of transcriptome and translatome identifies that cell cycle pathway gene MCM5 is a target of FIBCD1.

From: Diabetes is causally associated with increased breast cancer mortality by inducing FIBCD1 to activate MCM5-mediated cell cycle arrest via modulating H3K27ac

Fig. 6

A Schematic workflow showing RNA extraction from ribosome-protected mRNA fragments (RPFs) or from whole cell lysates in FIBCD1-overexpressing and control cells to be used for Ribo-seq or RNA-seq analyses. B Numbers of significantly up- or down-regulated transcripts shown as doughnuts. C Genome-wide transcriptional and translational regulations show very little correlation. E Heatmaps representing the top 30 up- or downregulated transcripts in the translatome and transcriptome with |fold change>2.0| and P-value < 0.05. D The four-way Venn diagram showing the overlap between the significantly upregulated or down-regulated transcripts in the translatome and transcriptome. F Volcano map of differentially expressed TE genes (DTEG). The blue dot represents the non-differentially expressed translation efficiency genes. G Gene set enrichment analysis (GSEA) of DTEG showing the enrichment of Cell cycle pathway in FIBCD1-overexpressing cells, with MCM5 as one of the top up-regulated genes. H GSEA analysis showing the enrichment of DNA replication pathway in FIBCD1-overexpressing cells, with MCM5 as one of the top up-regulated genes. I Overexpression/Knockdown of FIBCD1 promotes/inhibits MCM5 expression in MDA-MB-231 cells. Data are presented as mean ± SD, Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.

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