Fig. 7: FIBCD1 affects BC cell cycle and proliferation by interacting with MCM5 in a glucose level-dependent manner. | Cell Death & Disease

Fig. 7: FIBCD1 affects BC cell cycle and proliferation by interacting with MCM5 in a glucose level-dependent manner.

From: Diabetes is causally associated with increased breast cancer mortality by inducing FIBCD1 to activate MCM5-mediated cell cycle arrest via modulating H3K27ac

Fig. 7

A Overexpression of FIBCD1 arrests MCF7 cells in the S and G2/M phases. B FIBCD1 induces S and G2/M phases arrest in MDA-MB-231 cells. C Knockdown of FIBCD1 reduces the percentage of S phase cells. D FIBCD1 promotes Cyclin A1 and Cyclin D1 protein expression in both MCF7 and MDA-MB-231 cells. E Co-transfection of FIBCD1 and si-MCM5 partly reverses the impact of FIBCD1 on S phase arrest of cell cycle in hyperglycemic compared with euglycemic conditions in MDA-MB-231 cells. F si-MCM5 partially abolishes the effect of FIBCD1 on the percentage of S phase cells in hyperglycemic compared with euglycemic conditions in MCF7 cells. G Co-transfection of FIBCD1 and si-MCM5 suppresses the influence of FIBCD1 on cell proliferation rate in hyperglycemic compared with euglycemic conditions in MDA-MB-231 cells. H si-MCM5 inhibits the promotive effects of FIBCD1 on cell proliferation in hyperglycemic compared with euglycemic conditions in MCF7 cells. Data are presented as mean ± SD, Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.

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