Fig. 7: The upregulation of SUMOylation in response to MLN4924 treatment requires p14ARF.
From: SUMOylation of the lysine-less tumor suppressor p14ARF counters ubiquitylation-dependent degradation
A PC3 cells were treated with DMSO or MLN4924 and, at different times after treatment, UBC9, SUMO1, and SUMO2 were analyzed by QRT-PCR. Values were normalized to GAPDH expression. Columns are representative of the mean and error bars represent the SD of three biological replicas. Statistical analysis was assessed by Student’s t-test. **P < 0.005; ***P < 0.0005. B PC3 or PC3-shp14ARF cells were treated with MLN4924 or DMSO and 48 h after treatment, protein extracts were analyzed by western-blot with the indicated antibodies. C PC3 or PC3-shp14ARF cells were treated with DMSO or MLN4924 and 48 h after treatment, transcription of UBC9, SUMO1, and SUMO2 were analyzed by QRT-PCR. Values were normalized to GAPDH expression. Columns are representative of the mean and error bars represent the SD of six biological replicas. Statistical analysis was assessed by Student’s t-test. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001. hpt hours post-treatment.
