Fig. 1: circPSD3 Up-regulation in Thyroid Carcinoma.

A Volcano plot displaying differentially expressed circular RNAs between 5 PTC tissues and adjacent normal thyroid tissues. B (Upper panel) Venn diagram showing a set of 5 upregulated circular RNAs in 2 PTC databases. (Lower panel) Relative expression levels of the 5 circular RNAs in thyroid epithelial cell line (Nthy-ori 3-1) and TC cell lines (TPC-1, BCPAP, 8305 C). C (Lower panel) Schematic circular structure of circPSD3 and its junction sequence, verified by PCR and Sanger sequencing (Upper panel). D Upregulated levels of circPSD3 in PTC tissues were verified in our validation cohort (n = 21). E, F RNA hybridization in situ illustrating different expression levels of circPSD3 in thyroid carcinoma tissues with or without lymph node metastasis (F). Staining score of circPSD3 was calculated, indicating a higher circPSD3 expression level in tissues with lymph node metastasis (E). G Relative RNA levels of linear PSD3 and circPSD3 after treatment with or without RNaseR. H Electropherogram displaying the PCR amplification products by divergent primers or convergent primers. I Relative RNA levels of circPSD3 in nuclear and cytoplasmic fractions from TPC-1 and 8305 C. J Subcellular localization of circPSD3 in TPC-1 and 8305 C detected by FISH. The experiments were repeated 3 times independently. Normally distributed data are presented as the mean ± S.D, non-normally distributed data are presented as median (95%CI). cDNA complementary DNA, gDNA genomic DNA. *P < 0.05; **P < 0.01; ***P < 0.001; ns no significance.