Fig. 6: circPSD3 regulation of the TCA cycle and promotion of proliferation by Sponging miR-338-5p. | Cell Death & Disease

Fig. 6: circPSD3 regulation of the TCA cycle and promotion of proliferation by Sponging miR-338-5p.

From: CircPSD3 aggravates tumor progression by maintaining TCA cycle and mitochondrial function via regulating SUCLG2 in thyroid carcinoma

Fig. 6

A, B Flow cytometry and statistical results revealed the miR-338-5p inhibitor reversed the dropping MMP in the sh-circPSD3 group in TPC-1. C, D Flow cytometry and statistical results revealed the miR-338-5p inhibitor reversed the increased apoptotic cell rate in the sh-circPSD3 group in TPC-1. E, F Representative EdU fluorescence images and statistical results revealed the miR-338-5p inhibitor reversed the inhibitory effect on proliferation in the sh-circPSD3 group in TPC-1. G, H The suppressed mitochondrial respiration and ATP production in the sh-circPSD3 group were rescued by the miR-338-5p inhibitor in TPC-1. I Subcutaneous xenograft tumors after multiple intratumoral injections with siNC, si-circPSD3, si-circPSD3+NC-inhibitor, si-circPSD3+miR-338-5p inhibitor. Tumor growth curves (I), gross appearance of tumors taken on the same scale (J), and tumor weight analysis (K) of TPC-1. The experiments were repeated 3 times independently. Data are presented as the mean ± S.D. miR-NC miRNA inhibitor negative control, miR-I hsa-miR-338-5p inhibitor, NC negative control group, sh sh-circPSD3 group. *P < 0.05; **P < 0.01; ***P < 0.001.

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