Table 1 Diversity in the Composition of NETs.
Neutrophil origin | NETs induction | DNase/MNase treatment | Method | Identified proteins | Reference |
|---|---|---|---|---|---|
Human, healthly | 20 mM PMA, 4 h | DNase I | nano LC-MALDI-MS | NE, LTF, AZU1, CatG, MPO, PR3, LYZ, DEFA1, DEFA3, H2AJ, H2BC21,H2BC12L, H3C15, H4C1, MNDA, S100A8, S100A9, S100A12, ACTB, ACTG1, MYH9, ACTN1, ACTN4, LCP1, KRT10, CAT, ENO1, TKT | Urban et al. [7] |
Human, healthly | 50 mM PMA, 3 h | MNase | LC-MS/MS | ACTB, MPO, LTF, NE, HIST2H2BF, H3F3B, HIST1H2AH, AZU1, HIST1H4L, MYH9, TKTL1, ENO1, HIST1H1E, LYZ, CatG, S100A9, DEFA1, GAPDH, MNDA-like, GSN, CLC, GPI, PFN1, CORO1A, PGK1, PKM, BASP1, VIM, CFL1, TMSB4X, RNASE3, S100A8, PPIA, GSTP1, TALDO1, Granulins-like, PGD, CAMP, GMFG, HMGB2, LSP1-like, S100A12, Plastin-2-like, ANXA1, TPM2, SH3BGRL3, HMGN2, HP1BP3, MMP9-like, DKFZp686B04128 | O’Donoghue et al. [3] |
Human, healthly | P. auerginosaa, 100 min | MNase | LC-MS/MS | TALDO1, TKT, GPI, ALDOA, TPI1, ENO1, PGK1, GAPDH, LDHB, LDHA, PGAM1,MDH1, MDH2, CORO1A, ACTA2, ACTN1, ACTB, PFN1, MYL6B, ARPC1B, GSN, ACTN4, MSN, ACTR3, CAPZA1, HIST1H4A, HIST1H2BA, HIST1H2BB, HIST3H3, HIST1H2BJ, HIST1H2AB, HIST1H2BC, HIST1H3A, HIST1H1A, HIST1H2AD,HIST1H1C, LTF, ERPINB1, MPO, LCN2, LYZ, CAMP, NE, PSMA1, ANXA1, ANXA3, PPIA, HSPA8, HSPA1A, HSPA1L, MMP9, HSPE1, HSPA5, ANXA5, CAT, PRDX2, PDIA3, PRDX1, SOD1, UBA52, S100A12, RETN, FTL, YWHAG, FTH1, PDIA3, YWHAE, SET, ANP32A, NAA38, S100A4, ARHGDIB | Dwyer et al. [152] |
Human, CF sputum | P. auerginosa | DNase I | LC-MS/MS | GAPDH, TPI1, CORO1A, MYH9, GSN, PFN1, ACTN1, FLNA, ACTN4, LTF, MPO, EPX, CatG, PGLYRP1, AZU1, CAMP, NE, LYZ, S100A12, S100A4, BPIFB2, ANXA1, ANXA3, ANXA4, HSPA8, ANXA6, HSPA1A, HSPA1L, HSPA2, HSPA5, CAT, PR3, PLBD1, CHI3L1, MMP8, MMP9, SERPINA3, PPIB, QSOX1, SERPINB10, SERPING1, SERPINA1, TKT, TALDO1, VCL, ITGAM, AZGP1, C3, LRG1, CRISP3, HPX, IQGAP1, LCP1, A1BG, MUC5B, LCN2, CAP1 | |
Human, healthly | 100 mM PMA, 3 h | DNase I | nano-UHPLC-MS/MS | ACTR3C, STX1A, CS, OGDH, SYNJ1, NXN, HSPA12A, ASAH1, KRT10, ADD2, NAP1L4, NCOA1, GRIA2, CALB1, RAB1B, SKP1, GPD2, SLC8A2, PACSIN1, DLST, EIF3G, CLIC1, TPPP, CA2, GNAI1, ATP1A1, SLC25A6, AKR1A1, CDH2, COX5A, UBA1, HSPA9, RBM11, AKAP6, SHB, SETX, PKD1L2, HIST2H2AB b | Petretto et al. [154] |
1 µg/ml LPS, 3 h | SIRPA, EPX, ORM1, HAUS5, HUS1, PRPF40B, CCDC154 b | ||||
4 µM A23187, 3 h | ATAD5, GPI, IQGAP1, ELP3, MYL6, WDR1, CHIT1, NLN, ANXA5, CEP112, UACA, COPG1, UNC13D, MAN2B1, AKAP8, CSTB, UBTFL1, SPTB, HSP90B2P, CAPZA1, DST, CLLU1OS, TMEM69, BSPRY, RAD50, ZNF251, AKAP8 b | ||||
Human, healthly | 25 mM PMA, 4 h | No | FRET enzyamtic activity (NE, CG, PR3), MPO activity, flow cytometry (cytokine panel) | NE, CatG, PR3, MPO, IL-8, IL-1RA, G-CSF, IL-1α, IL-1β, TNF-α | Collins et al. [153] |
