Fig. 2: Dispersion of NuMA and duplicated chromosomes in mitotic spindles of multi-centrosomal cancer cells.
From: The post-translational modification of NuMA in cancer cells is a new target for cancer eradication
Left panel: A schematic presentation of a normal spindle of an untreated cancer cell in mitosis (control), and an aberrant spindle of PJ34-treated cancer cell. In the treated cancer cell, un-clustered NuMA in the spindle poles, dispersed centrosomes and dispersed duplicated chromosomes, leading to activation of the spindle assembly control (SAC) mechanism, mitosis arrest and cell death. Microtubules (green), centrosomes (red), NuMA (purple) and duplicated chromosomes (blue) are displayed. Right panel: multi-centrosomal MDA-MB-231 cells. Untreated cell (control): A confocal image of clustered centrosomes (immune-labeled, red) in the pole of a well-constructed spindle, and chromosomes aligned in the spindle mid-zone (DAPI-labeling, blue). PJ34 treated cells: confocal images of aberrant spindle poles with scattered chromosomes (DAPI-labeling, blue) un-aligned in the spindle mid-zone, and dispersed centrosomes (immuno-labeled, red). Microtubules are labeled by Immuno-labeled α-tubulin (green). From: [15] and [18].
