Fig. 3: P300/CBP and class I HDAC regulated neuronal ferroptosis by affecting H3K14la levels in vitro.

A After hemin treatment of PCNs at 12 h, the expression of H3K14la was detected after 50 µM hemin treatment and combined administration with A485 or Apicidin (left side: n = 4 cultures; right side: n = 8 cultures). B, C The changes in cell death after 50 µM hemin and A485 or Apicidin combined treatment later 24 h were detected by PI and Hoechst staining (B: n = 3 cultures; C: n = 3 cultures). D, E BODIPY 581/ 591 C11 reagent was used to detect the changes of intracellular lipid ROS later at 24 h after treatment with 50 µM hemin and A485 or Apicidin. n = 5 cultures. F The cell death of PCNs transduced with the AAV-hSyn-EGFP-NC (NC) or AAV-hSyn-EGFP-shP300 (shP300) at 24 h after hemin treatment was detected by PI and Hoechst staining. n = 3 cultures. G BODIPY 581/591 C11 reagent was used to detect the changes of intracellular lipid ROS in PCNs transduced with AAV virus NC or shp300 at 24 h after hemin treatment. Results are shown as scatter plots (Mean ± SD). n = 3-4 cultures. One-way ANOVA followed by Tukey’s multiple comparisons tests was used. *p < 0.05, ***p < 0.001 vs Veh; #p < 0.05, ##p < 0.01, ###p < 0.001 vs Hemin.