Fig. 7: JQ-1-PROTAC reduces erastin-induced death in H9C2 cardiomyocytes. | Cell Death & Disease

Fig. 7: JQ-1-PROTAC reduces erastin-induced death in H9C2 cardiomyocytes.

From: (+)-JQ-1 alleviates cardiac injury in myocardial infarction by inhibiting ferroptosis through the NAMPT/SIRT1 pathway

Fig. 7

A JQ-1-PROTAC Chemical formula. The Figure was created with ChemDraw software (https://www.chemdraw.com.cn/). B Effect of JQ-1-PROTAC (0.5 μM) on the cell viability in H9C2 cardiomyocytes under the erastin challenge. JQ-1 and Fer-1 were used as positive controls (n = 6). C Representative images of Calcein-AM and PI staining in H9C2 cardiomyocytes treated with JQ-1-PROTAC under the erastin challenge. Green indicates the fluorescence of Calcein-AM, red indicates the fluorescence of PI. Scale bars, 100 µm, (n = 6). D Representative DCFH-DA fluorescence (ROS probe) images in H9C2 cardiomyocytes treated with JQ-1-PROTAC under the erastin challenge. Scale bars, 100 µm. E Quantitative analysis of ROS levels in (D) (n = 4). F Representative lipid peroxidation staining images in H9C2 cardiomyocytes treated with JQ-1-PROTAC under the erastin challenge. Red: unoxidized C11-BODIPY; Green: oxidized C11-BODIPY; Blue: Hoechst-stained nucleus. Scale bars, 50 μm. G Quantitative analysis of lipid peroxidation levels in (F) (n = 3). Quantitative analysis of GSH/GSSG levels (H) and MDA levels (I) in H9C2 cardiomyocytes with JQ-1-PROTAC treatment under the erastin challenge (n = 3). J Western blot analysis of the GPX4 protein levels in H9C2 cardiomyocytes with JQ-1-PROTAC treatment under the erastin challenge. K Quantitative analysis of relative GPX4 protein levels in (J) (n = 4). The data are presented as mean ± SEM. The statistical significance of the data was evaluated using a two-way ANOVA followed by Tukey’s test for multiple comparisons (B, E, G–I, and K). **P < 0.01.

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