Fig. 8: JQ-1 reduces erastin-induced death in human cardiomyocytes. | Cell Death & Disease

Fig. 8: JQ-1 reduces erastin-induced death in human cardiomyocytes.

From: (+)-JQ-1 alleviates cardiac injury in myocardial infarction by inhibiting ferroptosis through the NAMPT/SIRT1 pathway

Fig. 8

A Effect of JQ-1-PROTAC on the cell viability in AC16 cardiomyocytes under the erastin challenge (n = 6). B Representative fluorescence images of DCFH-DA staining in AC16 cardiomyocytes treated with JQ-1-PROTAC under the erastin challenge. Scale bars, 100 µm. C Quantitative analysis of ROS levels in (B) (n = 3). D Representative lipid peroxidation images in AC16 cardiomyocytes treated with JQ-1-PROTAC under the erastin challenge. Red: unoxidized C11-BODIPY; Green: oxidized C11-BODIPY; Blue: Hoechst-stained nucleus. Scale bars, 50 μm. E Quantitative analysis of lipid peroxidation levels in (D) (n = 3). F Schematic diagram of JQ-1 protects against MI-induced cardiomyocytes ferroptosis through NAMPT-SIRT1 signaling. NA nicotinic acid, NAPRT NA phosphoribosyltransferase, NaMN nicotinic acid mononucleotide, NAD+ nicotinamide adenine dinucleotide, NAM nicotinamide, NMN nicotinamide mononucleotide, NAMPT nicotinamide phosphoribosyl-transferase, PUFA polyunsaturated fatty acid. Created with BioRender.com. The data are presented as mean ± SEM. The statistical significance of the data was evaluated using a two-way ANOVA followed by Tukey’s test for multiple comparisons (A, C, and E). **P < 0.01.

Back to article page