Fig. 5: HIF-1α alleviated DSS-induced acute colitis in mice.
From: HIF-1α alleviates ferroptosis in ulcerative colitis by regulation of GPX4
A Intervention methods and cycles of DMOG, 2-ME2, RSL3, and Fer-1 in C57BL/6 male mice. B Appearance characteristics of mice after normal control (NC) group and 3% DSS intervention. Red arrow indicates blood residue after defecation. C Immunohistochemical staining of MPO in colonic sections (Scale: 100 μm, 50 μm). D, E Changes of murine colon length and weight after different intervention. F Disease activity index (DAI) scores of mice. G Expression levels of inflammatory factors IL-1β, IL-6, and TNF-α were detected in mice serum by ELISA kit. H H&E staining of colon tissues of mice (Scale: 200 μm, 100 μm). I, J Immunohistochemical staining of Occludin, ZO-1, and HIF-1α in different groups (Scale: 200 μm, 100 μm). K Western blot analysis of ZO-1, Occludin, and HIF-1α in the mice colon tissues. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
