Fig. 6: Inhibition of ferroptosis alleviated DSS-induced acute colitis in mice.
From: HIF-1α alleviates ferroptosis in ulcerative colitis by regulation of GPX4
A, B Changes of murine colon length and weight after DSS and Fer-1 intervention. C Disease activity index (DAI) scores of mice. D Expression levels of inflammatory factors IL-1β, IL-6, and TNF-α were detected in mice serum by ELISA kit. E, F Transmission electron micrographs (TEM) and H&E staining of murine colon tissues in different groups. G MDA levels in the colonic tissues of mice in each group using the MDA kit. H Western blot analysis of FTL and FTH with β-actin as control. I–K Immunofluorescence staining of HIF-1α, FTH, and CK18. Nuclei were stained with DAPI in blue, HIF-1α localization was indicated in red, FTH localization was indicated in green, CK18 staining was indicated in gray (Scale: 100 μm). L Determination of iron content of intestinal epithelial cells in mouse colon tissues using iron assay kit. M, N The protein and mRNA levels of ferroptosis key genes (ACSL4, SLC7A11, GPX4, LPCAT3, and TXNRD1) and HIF-1α in colon tissues of NC and DSS-induced acute colitis mice. O Immunohistochemical staining of LPCAT3, ACSL4, GPX4, TXNRD1, and SLC7A11 in colon tissues of normal control (NC) and DSS-induced acute colitis mice. ns P> 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
