Fig. 9: YY1 enhances the transcriptional expression of USP43. | Cell Death & Disease

Fig. 9: YY1 enhances the transcriptional expression of USP43.

From: YY1-induced USP43 drives ferroptosis suppression by FASN stabilization and subsequent activation of SLC7A11 in ovarian cancer

Fig. 9

A Common transcription factors of USP43 predicted using four databases. B Correlation analysis between YY1 and USP43 expression levels in OC using TCGA. C Overexpression of YY1 increases USP43 mRNA levels (n = 3). D Knockdown of YY1 decreases USP43 mRNA levels. E JASPAR prediction of YY1-binding motif regions (n = 3). F ChIP-qPCR validation of YY1 bound to the predicted USP43 promoter region (n = 3). G Construction of full-length USP43 promoter sequence and truncations. H Validation of YY1 binding to the USP43 promoter region using luciferase assay (n = 3). I A simplified working model depicting YY1-activated USP43, which facilitates ferroptosis suppression in OC cells by inducing SLC7A11 transcription. USP43 binds to and stabilizes FASN via its deubiquitinating enzyme activity. Subsequently, the stabilized FASN maintains HIF1α levels, promoting its accumulation in the nucleus. Finally, nuclear HIF1α enhances the transcription of SLC7A11, aiding OC cells in resisting ferroptosis. Data are presented as mean ± SD.

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