Fig. 2: Knockout of PRMT1 in tubular epithelial cells can alleviate calcium oxalate-induced renal injury by improving fatty acid metabolism.

A Representative histology images of the indicated groups: Vonkossa staining indicates the area and location of calcium oxalate crystals deposition (400×); HE staining shows the degree of renal tubular injury (200×); TUNEL staining shows the relative number of apoptotic renal cells (200×). Scale bars = 50 μm. B, C Representative TEM images demonstrate the degree of mitochondrial damage and lipid droplet accumulation. Scale bars = 500 nm. D Representative western blot banding and quantitative analysis of the expression levels of ACOX1, CPT1a, CD36, and FATP2. E Mitochondrial oxidative capacity was measured in real time after knockdown of PRMT1 or COM treatment in HK-2 cells; Quantification of basal respiration, ATP production-coupled respiration, and maximal respiration. Significance was assessed using two-way ANOVA tests. Data are shown as mean ± SD.