Fig. 3: Overexpression of PRMT1 in tubular epithelial cells can aggravate calcium oxalate-induced renal injury by inhibiting fatty acid metabolism.

A Representative histology images of the indicated groups: Vonkossa staining indicates the area and location of calcium oxalate crystal deposition (1X and 200X); HE staining shows the degree of renal tubular injury (200X); TUNEL staining shows the relative number of apoptotic renal cells (200X). Scale bars = 50 μm. B, C Representative TEM images demonstrate the degree of mitochondrial damage and lipid droplet accumulation. Scale bars = 500 nm. D Representative western blot bandings show the expression levels of ACOX1 and CPT1α. E Mitochondrial oxidative capacity was measured in real time after overexpression of PRMT1 or COM treatment in HK-2 cells; basal respiration, ATP production-coupled respiration, and maximal respiration were quantified. Significance was assessed using two-way ANOVA tests. Data are shown as mean ± SD.