Fig. 6: PRMT1 regulates NEDD4-mediated PPARγ ubiquitination by methylating UBE2m in tubular epithelial cells. | Cell Death & Disease

Fig. 6: PRMT1 regulates NEDD4-mediated PPARγ ubiquitination by methylating UBE2m in tubular epithelial cells.

From: PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism

Fig. 6

A RT-PCR was used to detect the RNA levels of PPARγ from the indicated groups. Representative western blot banding and quantitative analysis show the expression levels of PPARγ from the indicated groups in mice (B) or in HK-2 cells (C). D Detection of endogenous ubiquitination levels of PPARγ in HK-2 cells with COM-treatment. E Detection of endogenous ubiquitination levels of PPARγ in CaOx crystals kidney injury in mice. F, G Detection of exogenous ubiquitination levels of PPARγ in HEK193T cells with PRMT1 knockdown or overexpression. H Detection of exogenous ubiquitination levels of PPARγ in HEK193T cells from the indicated groups. Representative western blot banding and quantitative analysis of the expression levels of NEDD4 from the indicated groups in mice (I) or HK-2 cells (J). K HK-2 cells were treated with MG132. Representative western blot banding and quantitative analysis showed the expression levels of PPARγ from the indicated groups. L PLA detection of NEDD4 and PPARγ interaction in HK-2 cells from the indicated groups. Scale bars = 50 μm. M Detection of exogenous ubiquitination levels of PPARγ in HEK193T cells with knockdown of NEDD4. N Detection of exogenous ubiquitination levels of PPARγ in HEK193T cells from the indicated groups. Significance was assessed using two-way ANOVA tests. Data are shown as mean ± SD.

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