Fig. 8: USP14 and UCHL5 promote the malignant phenotype of ATC cells by PKCα.

A Colony formation assays were performed to assess the proliferation of cells co-transfected with siRNAs targeting USP14 and UCHL5, alongside PKCα plasmids. The experiment was repeated three times. B Wound healing assays illustrating the migration capability of CAL62 and 8505 C cells co-transfected with siRNAs targeting USP14 and UCHL5, as well as PKCα plasmids, at 0 and 24 h. Scale bar, 400 μm. The experiment was repeated three times. C Transwell assays demonstrating the impact of co-transfection with siRNAs targeting USP14 and UCHL5, along with PKCα plasmids, on the migration and invasion capability of CAL62 and 8505 C cells. Scale bar, 400 μm. The experiment was repeated three times.