Fig. 3: USP24 inhibitor exhibits a synergistic effect with ferroptosis inducer in TNBC cells. | Cell Death & Disease

Fig. 3: USP24 inhibitor exhibits a synergistic effect with ferroptosis inducer in TNBC cells.

From: The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein

Fig. 3

A Cell viability of MDA-MB-231 cells treated with the indicated concentrations of RSL3 in combination with WP1130, and the results was analyzed by SynergyFinder web-application. ZIP synergy score>10 indicates a synergistic effect between the two agents. B Cell death was assessed using propidium iodide (PI) staining in MDA-MB-231 and MDA-MB-468 cells treated with the indicated concentrations of RSL3 in the presence or absence of WP1130 (0.5 μM) for 24 h. Quantification of PI-positive cells was shown. Mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns no significance. C Lipid peroxidation of MDA-MB-231 and MDA-MB-468 cells treated with RSL3 (0.5 μM for MDA-MB-231 cells; 0.25 μM for MDA-MB-468 cells) in the presence or absence of WP1130 (0.5 μM) for 6 h. Mean ± SD, n = 3. **P < 0.01, ***P < 0.0001, ****P < 0.0001. Migration (D) and cloning formation (E) abilities of MDA-MB-231 cells and MDA-MB-468 cells treated with RSL3 (0.5 μM for MDA-MB-231 cells; 0.25 μM for MDA-MB-468 cells) in the presence or absence of WP1130 (0.5 μM) for 6 h. Mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. F, G The representative phase-contrast and propidium iodide (PI) staining images of MDA-MB-231 three-dimensional (3D) spheroids treated with the indicated doses of RSL3 in the presence or absence of WP1130 (0.5 μM) for 48 h. The relative PI fluorescence intensity was quantified as the ratio of red fluorescence intensity (PI) to blue fluorescence intensity (Hoechst 33342). Mean ± SD, n = 3. *P < 0.05, **P < 0.01. ns no significance.

Back to article page