Fig. 2: Vesicles released from CRC cells upon P2X7 stimulation increase cell dissemination and metastasis formation.
A Quantification of the migration capacity of CT26 cells alone or incubated with S-VS, P2X7-VS, and AZ-VS. The migration ability is expressed as the percentage of the area covered by cells evaluated 24 and 48 h after the scratch formation (n = 3). Mice were intravenously injected with CT26 Luc2 cells alone or pre-treated with 4 × 109 S-VS, P2X7-VS, or AZ-VS. The vesicles were obtained from batches of cells different from those injected; see materials and methods. B Representative images of luminescence emission on day 14 from the inoculum. C Quantification of photon emission, expressed as a total flux, at day 14 (n = 8). D The area of the lung covered by metastasis is expressed as a percentage of the total lung area (n = 5). E Representative hematoxylin/eosin staining of mouse lungs. F Number of particles measured in the mice’s plasma at day 14 (n = 5). Systemic level of the proinflammatory cytokines IL-17 (G, n = 8). H Representative Western blot of FOXP3 expression in mice’ lungs. I Densitometric analysis of FOXP3. Data were normalized on myosin (n = 3). *p < 0.05, **p < 0.001, ***p < 0,0001, ****p < 0.00001.
