Fig. 6: Double antagonism of P2X7 and A2A significantly reduces tumor growth in a syngeneic subcutaneous CRC model but is not efficacious in immunocompromised nude mice.

BALB/c mice were subcutaneously injected with CT26 and treated with placebo, AZ10606120 (2 mg/Kg), SCH58261 (1 mg/Kg) or both drugs (n = 12). A Ex vivo tumor volume on day 14, and B representative images of tumors. C Systemic levels of the proinflammatory cytokine IL-17 (n = 4). nude/nude mice were subcutaneously injected with HCT116 cells and treated i.p. with placebo, AZ10606120 (2 mg/Kg), SCH58261 (1 mg/Kg) or both drugs. D Ex vivo tumor volume at day 14 from the inoculum (n = 6). E Representative tumor image. F HCT116 Luc2 were injected into the tail vein of nude/nude mice and treated with placebo, AZ10606120 (2 mg/Kg), SCH58261 (1 mg/Kg) or both drugs (n = 7). Photon emission on day 14 total flux (p/s). G Image representative of data shown in F. *p < 0.05, **p < 0.001.