Fig. 2: Serum Prdx1 was increased in mice with experimental colitis. | Cell Death & Disease

Fig. 2: Serum Prdx1 was increased in mice with experimental colitis.

From: Peroxiredoxin 1 promotes intestinal inflammation by activating the NLRP3 inflammasome in macrophages through lysosomal disruption in Crohn’s disease

Fig. 2

Wild-type mice were treated with 4% DSS for the indicated time (n = 5 mice per group). A Typical images of H&E staining and histopathological scores of colon sections from control mice and those treated with DSS for varying durations. Scale bar, 50 μm. B Immunofluorescence staining for Prdx1 in colon sections from the indicated mouse groups. Typical images are shown. Scale bar, 200 μm. C, D Prdx1 mRNA and protein expression in colon tissues from the indicated mouse groups were measured by RT-qPCR and western blot, respectively. Typical images and quantification are shown. E Serum Prdx1 levels in the indicated mouse groups were measured by ELISA and quantified using a standard curve. F The linear correlation between serum Prdx1 levels and disease activity index (DAI) scores in mice was analyzed by Pearson’s correlation coefficient. G–I The linear correlations between serum Prdx1 levels and levels of serum IL-1β, IL-6, and TNF-α were analyzed by Pearson’s correlation coefficient. Data are expressed as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by non-paired Student’s t test. Results presented were repeated at least three independent experiments for each experimental condition.

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