Fig. 6: Serum Prdx1 induced intestinal inflammation by enhancing macrophage infiltration and interacting with macrophages.

A Immunofluorescence staining for F4/80 in colon sections from Prdx1^–/– mice and their littermates treated with DSS (n = 5 mice per group). Typical images and quantification are included. Scale bar, 20 μm. B Immunofluorescence staining for F4/80 in colon sections from DSS-treated Prdx1^–/– mice along with intravenous (i.v.) injection of PBS or rPrdx1 (n = 5 mice per group). Typical images and quantification are included. Scale bar, 20 μm. C Immunofluorescence staining for F4/80 in colon sections from DSS-treated WT mice along with intraperitoneal (i.p.) injection of control IgG or Prdx1-neutralizing antibody (n = 5 mice per group). Typical images and quantification are included. Scale bar, 20 μm. D–K WT mice were depleted of macrophages using clodronate liposomes (CLs) and subsequently administered 3% DSS, accompanied by i.v. injection of either PBS or rPrdx1 (10 μg/kg; n = 5 mice per group). D Schematic diagram of DSS-treated mice with macrophage depletion. E, G Immunofluorescence staining for F4/80 in colon sections from the indicated mouse groups. Typical images and quantification are included. Scale bar, 20 μm. F, H Typical images of H&E staining and histopathological scores of colon sections from the indicated mouse groups. Scale bar, 50 μm. I–K The mRNA expression levels of IL-1β, IL-6, and TNF-α in the colons of indicated mouse groups were measured by RT-qPCR. Data are expressed as mean ± SD; n = 5 mice per group. ^*P < 0.05, ^**P < 0.01 by one-way ANOVA. Results presented were repeated at least three independent experiments for each experimental condition.