Fig. 7: Prdx1 activates the NLRP3 inflammasome in macrophages.

A–E Primary peritoneal macrophages (PPMs) isolated from wild-type mice were stimulated with rPrdx1 (25 nM) for 12 and 24 hours (h) with or without pretreatment of MCC950 (10 μM for 1 h). Cells were collected and analyzed for the expression levels of pro-caspase-1 (pro-Casp1) (A), NLRP3 (B), and pro-IL-1β (C). Conditioned medium was harvested and analyzed for cleaved Casp1 (D) and mature IL-1β (E) expression levels. Typical images and quantification are included (n = 3 biological repeats). F, G NLRP3 protein expression and quantification in colon tissues from Prdx1^–/– mice and their littermates treated with DSS (n = 6 mice per group). H IL-1β levels in colon tissues from the indicated mouse groups were detected by ELISA (n = 6 mice per group). I–K WT mice were depleted of macrophages using clodronate liposomes (CLs) and subsequently administered 3% DSS, accompanied by i.v. injection of either PBS or rPrdx1 (10 μg/kg; n = 5 mice per group). NLRP3 protein expression and quantification (I, J), along with IL-1β levels (K) were detected in colon tissues from the indicated mouse groups. Data are expressed as mean ± SD; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 by one-way ANOVA. Results presented were repeated at least three independent experiments for each experimental condition.