Fig. 8: Cell-free Prdx1 induces lysosomal disruption to activate the NLRP3 inflammasome. | Cell Death & Disease

Fig. 8: Cell-free Prdx1 induces lysosomal disruption to activate the NLRP3 inflammasome.

From: Peroxiredoxin 1 promotes intestinal inflammation by activating the NLRP3 inflammasome in macrophages through lysosomal disruption in Crohn’s disease

Fig. 8

A PPMs isolated from Prdx1–/– mice were stimulated with rPrdx1 at 25 nM for 12 and 24 hours (h). Immunofluorescence staining was performed to detect Prdx1 (red) and Lamp2 (green) in PPMs, and typical images are shown. Scale bar, 20 μm. B PPMs from WT mice were preincubated with fluorescent dextran and then stimulated with rPrdx1 at 25 nM for 3, 6, or 12 h. Immunofluorescence staining for dextran was conducted, and typical images are shown. Scale bar, 10 μm. C, D PPMs from WT mice were stimulated with rPrdx1 (25 nM) for 3, 6, or 12 h, with or without pretreatment of bafilomycin A1 (Baf A1, 100 nM for 1 h). C The levels of cathepsin D in the cytosolic fraction were measured and quantified. D Conditioned medium was harvested and analyzed for IL-1β levels by ELISA. Data are expressed as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA. Results presented were repeated at least three independent experiments for each experimental condition.

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