Fig. 9: NLRP3 inflammasome plays a critical role in facilitating Prdx1-mediated colitis. | Cell Death & Disease

Fig. 9: NLRP3 inflammasome plays a critical role in facilitating Prdx1-mediated colitis.

From: Peroxiredoxin 1 promotes intestinal inflammation by activating the NLRP3 inflammasome in macrophages through lysosomal disruption in Crohn’s disease

Fig. 9

Wild-type mice were administered 3% DSS for 7 consecutive days, accompanied by intraperitoneal (i.p.) injection of either PBS or MCC950 (20 mg/kg) every day. Mice were then intravenously (i.v.) injected with rPrdx1 on Days 4, 5, and 6 (10 μg/kg; n = 5 mice per group). A Schematic diagram of administration of MCC950 to DSS-treated mice. B Body weight of the mice was monitored daily, and the data was plotted as a percentage of the initial body weight on Day 0. C The disease activity was monitored and scored daily for each mouse. D Typical images of H&E staining and histopathological scores of colon sections from the indicated mouse groups. Scale bar, 50 μm. E, F NLRP3 protein expression and quantification (E), along with IL-1β levels (F), were detected in colon tissues from the indicated mouse groups. Data are expressed as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA. Results presented were repeated at least three independent experiments for each experimental condition.

Back to article page