Fig. 4: BAP31 is phosphorylated by PINK1 at Ser 142. | Cell Death & Disease

Fig. 4: BAP31 is phosphorylated by PINK1 at Ser 142.

From: BAP31 represses endoplasmic reticulum stress-mediated apoptosis and alleviates neurodegeneration in Parkinson’s disease

Fig. 4

A, B The protein expression of PINK1 in vivo and in vitro (n = 3). C String database. D–F Molecular docking. G, H Co-immunoprecipitation validates PINK1 interaction with BAP31 (n = 3). I Immunoprecipitation verifies the phosphorylation of BAP31 (n = 3). J Overexpression of PINK1 impacts BAP31 phosphorylation in PC12 cells (n = 3). K NetPhos 3.1 database analysis. L Comparative analysis of amino acid sequences around the site among different species. M Immunoprecipitation analysis in PC12 cells after co-transfection of BAP31 (WT, Ser142A and Ser216A) and PINK1 (n = 3). **P < 0.01.

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