Fig. 1: Platelet-derived exosomal LINC00183 is upregulated in CRC and exhibits clinical significance. | Cell Death & Disease

Fig. 1: Platelet-derived exosomal LINC00183 is upregulated in CRC and exhibits clinical significance.

From: Platelet-derived exosomal LINC00183 facilitate colorectal cancer malignant progression driven by histone lactylation through stabilizing ENO1

Fig. 1

A TEM analysis of the morphology of PLT-Exos. Scale bar, 200 nm. B NTA analysis of PLT-Exos. C Western blot analysis of exosomal markers (CD9, CD63, and TSG101) and Calnexin (negative control) in PLT-Exos. D Confocal microscopy images of PKH26-labeled exosomes internalized by HT29 and SW480 cells. Scale bar, 10 μm. E Heatmap illustrating differential lncRNA expression between PLT-Exos derived from CRC patients and healthy individuals. F Volcano plot indicating significant differences in lncRNA expression between PLT-Exos derived from CRC patients and healthy individuals. G PLT-Exos from CRC patients and healthy individuals showed different levels of expression for the top five lncRNAs that were elevated and downregulated. H, I HT29 and SW480 treated with or without Annexin V were incubated with CRC/PLT-Exos and cellular LINC00183 expression was measured by RT-qPCR. J LINC00183 expression between CRC tissues and normal colon samples in the TCGA database. K Overall survival (OS) in TCGA-CRC patients was analyzed using the Kaplan-Meier method based on the expression of LINC00183 (P = 0.033). L, M FISH-based analysis of LINC00183 expression in CRC tissues and matched normal tissues. Scale bar, 200 μm. N Prognostic analysis of LINC00183 in 93 CRC cases from our center. O, P FISH-based analysis of LINC00183 expression in CRC samples with different TNM stage. Scale bar, 200 μm. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance.

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