Fig. 7: LINC00183-mediated H3K18 lactylation promotes CRC progression by stimulating GDF15 expression. | Cell Death & Disease

Fig. 7: LINC00183-mediated H3K18 lactylation promotes CRC progression by stimulating GDF15 expression.

From: Platelet-derived exosomal LINC00183 facilitate colorectal cancer malignant progression driven by histone lactylation through stabilizing ENO1

Fig. 7

A, B ECAR measurements in HT29 cells following ENO1/LINC00183 knockdown/overexpression. C Lactate production assay results in HT29 cells following ENO1/LINC00183 knockdown/overexpression. D Schematic of glycolysis, highlighting the methods used in this study to inhibit lactate production and histone lactylation. E and F Western blot study of H3K18 lactylation and Pan Kla levels in CRC cells treated for 24 hours with varying doses of 2-DG or Oxamate. G The impact of LDH deficiency on Pan Kla and H3K18 lactylation was evaluated by Western blotting. H The impact of ENO1 deficiency on Pan Kla and H3K18 lactylation was investigated by Western blotting. I Analysis of Pan Kla and H3K18 lactylation in HT29 and SW480 cells following CRC/PLT-Exos co-culturing and LINC00183 overexpression. J Examination of H3K18 lactylation and Pan Kla in CCC-HIE-2 and CRC cell lines. K CUT&Tag tests on HT29 cells using H3K18la antibodies. H3K18la enrichment was found in many genes’ promoter regions (replicate 1). L KEGG database analysis for H3K18la-related genes. M Flow diagram showing H3K18la’s downstream target genes. N In the GDF15, HDAC10, and CXCL1 promoter regions, H3K18la peaks were found in both Cut&Tag datasets. O GDF15 mRNA and protein levels in CRC cells after co-incubation with PLT-Exos or overexpression of LINC00183. P GDF15 expression in HT29 and SW480 cells treated with varying amounts of 2-DG and oxamate was examined using Western blot technology. Q IF study of GDF15 expression in tissue microarrays comparing CRC samples to matched normal colon tissue. 200 μm scale bar. R Prognostic analysis of GDF15 in 93 CRC cases from our center. S and T GDF15-deficient CRC cells were tested for viability using the CCK-8 assay. U Results of colony formation assays conducted in GDF15-deficient CRC cells. V The Transwell migration assay was used to assess the GDF15-deficient CRC cells’ capacity for migration. W, X Correlation between H3K18la and GDF15 expression. Data are derived from IF analysis of CRC tissue microarrays.

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