Fig. 2: MCL-1 expression in bCAFs is linked to their secretion of VEGF-A. | Cell Death & Disease

Fig. 2: MCL-1 expression in bCAFs is linked to their secretion of VEGF-A.

From: MCL-1 as a molecular switch between myofibroblastic and pro-angiogenic features of breast cancer-associated fibroblasts

Fig. 2

A qRT-PCR of VEGF-A, FGF2, and ANGPT1 mRNA in bCAFsgCTRL or sgMCL-1 normalized on RPLPO mRNA expression. Mean and SEM of five independent experiments are represented as relative quantity of mRNA. Student t-test, ***P < 0.001, ns not significant. B VEGF-A quantification by ELISA in conditioned media (CM) from bCAFs after MCL-1 gene silencing (bCAFsgMCL-1) or not (bCAFsgCTRL). The bCAFs CM were generated during 72 h in EGM2 (Endothelial Cell Growth Medium-2) medium supplemented with 1% of FBS (n = 6). Student t-test, **P < 0.01. C qRT-PCR of VEGF-A, FGF2, and ANGPT1 mRNA in bCAFs treated or not by S63845 500 nM for 18 h normalized on RPLPO mRNA expression. Mean and SEM of three independent experiments are represented as relative quantity of mRNA. Student t-test, **P < 0.01, ns non-significant. D VEGF-A quantification by ELISA in CM of bCAFs treated or not by S63845 500 nM for 18 h. Results were expressed as concentration (pg/ml) for 200,000 cells (n = 5). Student t-test, **P < 0.01. E Negative correlation (Spearman correlation coefficient r = −0.7381; p value = 0.0458) between VEGF-A concentration level in bCAFs CM and MCL-1 protein level (relative to actin level) determined by western-blot analysis in 8 primary cultures of bCAFs between passage 2 and 4.

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