Fig. 1: PVL surgery and assessment, Exosomes isolation and identification. | Cell Death & Disease

Fig. 1: PVL surgery and assessment, Exosomes isolation and identification.

From: Exosomes from liver progenitor cells carrying JAG1 activate notch signaling to promote liver regeneration in PVL rats

Fig. 1

A Normal rat liver and PVL surgery. B a–d show liver images after surgery. e–h display liver specimens harvested after perfusion. C Hypertrophic Liver Tissue (HLT), Atrophic Liver Tissue (ALT). H&E and Ki67 were used to characterize liver repair following surgery. D Hepatocyte (HC, BRL-3A), BRL-3A cell line supernatant exosomes (CS-Exo), Liver tissue exosomes (LT-Exo). Immunoblotting of exosome markers. E TEM images of exosomes reveal oval-shaped membrane structures resembling red blood cells. F The NTA results for exosomes show a unimodal curve, with most diameters between 50 and 200 nm. G Green indicates DiO-stained exosomes, red represents the BRL-3A cell membrane stained with Dil, and blue shows DAPI-stained nuclei. a–d illustrate the uptake of CS-Exo (Exosomes from the supernatant of BRL-3A cells) using a standard fluorescence microscope, while figures e–h depict the uptake of Tissue-Exo (liver tissue exosomes) captured with a confocal fluorescence microscope.

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