Fig. 2: Liver tissue exosomes activated YAP-Notch Signaling promote liver regeneration.

Hypertrophic Liver Tissue (HLT), Atrophic Liver Tissue (ALT). Sham-E, 6 h-E, 24 h-E, and 48 h-E refer to exosomes from the hypertrophic side of liver tissue after surgery. A Bar chart of CCK8 cell proliferation activity B Bar chart depicting the statistical analysis of EdU-positive cells in Fig. C. C Images of EdU-positive BRL-3A cells were captured using fluorescence microscopy. D Fluorescence images of liver tissue labeled with DiO and Ki67, showing that exosomes are taken up by liver cells, appearing as green fluorescence. E Bar chart depicting the statistical analysis of the proliferation index for Ki67-positive cells in Fig. D. F Immunoblot bands of YAP-Notch signaling pathway proteins in hypertrophic and atrophic liver tissue after surgery. G Immunoblot bands of proteins in the BRL-3A cell line (12 h) and the hypertrophic liver tissue of PVL rats (24 h) after exosome treatment. Values are presented as the mean (X̅) ± standard error (SE), n = 3.