Fig. 3: Liver tissue exosomes carrying JAG1 activated Notch Signaling promote liver regeneration. | Cell Death & Disease

Fig. 3: Liver tissue exosomes carrying JAG1 activated Notch Signaling promote liver regeneration.

From: Exosomes from liver progenitor cells carrying JAG1 activate notch signaling to promote liver regeneration in PVL rats

Fig. 3

Hypertrophic Liver Tissue (HLT), Atrophic Liver Tissue (ALT), JAG1-Cy3 (exosomes blocked with JAG1 primary antibody and Cy3 fluorescent secondary antibody), 24h-E (PVL24h HLT Exosomes), and 24h-E-Ab (exosomes blocked with JAG1 antibody). A Immunoblot bands for JAG1 protein in liver tissue exosomes. B and C IF of JAG1 protein in the hypertrophic side of the liver tissue, focusing on the periportal and pericentral veins. DAPI (blue) stains nuclei, Sox9 (pink) is a cholangiocyte marker, HNF4α (green) is a hepatocyte marker, and red represents the target protein JAG1. D Fluorescent uptake images of JAG1 protein on liver tissue exosomes, captured with a fluorescence microscope, showing exosomes labeled in green (DiO) and red (JAG1-Cy3). E Line chart showing CCK8 cell proliferation. F Bar chart depicting statistical analysis of EdU-positive cells in Fig. G. G Images of EdU-positive BRL-3A cells captured using fluorescence microscopy. H Fluorescence images of liver tissues labeled with DiO and Ki67. I Bar chart depicting the statistical analysis of the proliferation index of Ki67-positive cells in Fig. H. J Immunoblot bands of proteins in the BRL-3A cell line (12 h) and hypertrophic liver tissue of PVL rats (24 h) after exosome treatment. Values are presented as the mean (X̅) ± standard error (SE), n = 3.

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