Fig. 5: YAP induces exosomes carrying JAG1 activated Notch Signaling promote liver regeneration. | Cell Death & Disease

Fig. 5: YAP induces exosomes carrying JAG1 activated Notch Signaling promote liver regeneration.

From: Exosomes from liver progenitor cells carrying JAG1 activate notch signaling to promote liver regeneration in PVL rats

Fig. 5

YAP-Exo refers to exosomes from the supernatant of BRL-3A cells treated with 2.5 μM TRULI, while YAP-Exo-Ab indicates YAP-Exo after blockade with JAG1 antibody. JAG1-Cy3 (Exosomes blocked with JAG1 primary antibody and Cy3 fluorescent secondary antibody). DAPI (blue) stains cell nuclei, Sox9 (pink) is a cholangiocyte-specific marker, HNF4α (green) is a hepatocyte-specific marker, and Ki67 (yellow) marks proliferating cells. A Sox9/YAP dual-IF images reveal YAP-positive nuclei in Sox9+ LPCs around the portal vein. B Sox9/Ki67/HNF4α triple-IF images show that (a) Sox9+ cells are hepatocytes, (b) proliferating cells are also hepatocytes, (c) Sox9+ cells are non-proliferating, and (d) the merged fluorescence image reveals that Sox9+HNF4α+ LPCs are non-proliferating hepatocytes. C Cell proliferation curves after treatment with various doses of TRULI. D IC50 curve of Verteporfin E Immunoblot bands in the BRL-3A cell line after treatment with 2.5 μM TRULI and Verteporfin. F Immunoblot bands in the supernatant exosomes of the BRL-3A cell line after treatment with 2.5 μM TRULI and Verteporfin. G Fluorescent uptake images of JAG1 protein on YAP-Exo, captured with a confocal fluorescence microscope, show exosomes labeled in green (DiO) and red (JAG1-Cy3). H Line chart of CCK8 cell proliferation activity after treatment with PBS, YAP-Exo, and YAP-Exo-Ab. I Fluorescence images of liver tissue labeled with Ki67 after treatment with PBS, YAP-Exo, and YAP-Exo-Ab. J Bar chart depicting the statistical analysis of the proliferation index for Ki67-positive cells in Fig. J. K Immunoblot bands in the BRL-3A cell line (12 h) and the hypertrophic liver tissue of PVL rats (24 h) after treatment with PBS, YAP-Exo, and YAP-Exo-Ab. Values are presented as the mean (X̅) ± standard error (SE), n = 3.

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