Fig. 3: ALDH2 influences mitochondrial biosynthesis to support leukaemia cell proliferation in vivo. | Cell Death & Disease

Fig. 3: ALDH2 influences mitochondrial biosynthesis to support leukaemia cell proliferation in vivo.

From: The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemia

Fig. 3

A Procedures for subcutaneous tumour model establishment in KG1a mice. KG1a cells transfected with the empty vector lentivirus or the ALDH2-overexpression vector were subcutaneously injected into NCG mice (5 × 106 cells per mouse). After 30 days, the cells were given an intraperitoneal injection of 60 mg/kg Ara-C and/or 7.5 mg/kg CVT-10216 or PBS (3 times a week) for an additional 10 days. B The appearance of subcutaneous tumour tissue in each mouse group. C Overall survival of each treated mouse (n = 4 mice per group). P-values were calculated with the log-rank test. **: p < 0.01. D Weights of the mice in each group. E, F Immunohistochemical staining and histograms of subcutaneous tumour tissue samples showing the coexpression proportions of ALDH2- and Mito-positive cells. G, H The volume curves (H) and weights (G) of the subcutaneous tumour tissue in each group are shown. I, J HE-stained sections (I) of the subcutaneous tumour tissue from each mouse group and immunohistochemical staining of ALDH2, MT-CO1, MT-ND5 and MT-ATP6 (J) in the subcutaneous tumour tissue sections (each group of 20 images was analysed, n = 4 mice per group).

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