Fig. 1: Impaired carboxypeptidase-like trimming activity of γ-secretase in a selected population of neurons.
From: A unique subpopulation of wild-type neurons recapitulating familial Alzheimer’s disease phenotypes
A A schematic presentation of the C99 YPet-mTurquoise-GL (C99 Y-T) and its near-infrared analog, the C99 miRFP720-miRFP670 (C99 720-670) biosensors for recording endogenous γ-secretase activity. B Endogenous γ-secretase activity is recorded and visualized using the C99 Y-T FRET biosensor. Scale bar 100 μm. C Quantification and histogram show that endogenous γ-secretase activity is heterogeneously regulated on a cell-by-cell basis. D 20% of neurons displaying the highest and lowest γ-secretase activity are sorted out using FACS, and (E) their cell lysates were subjected to human Aβ 38 and 42 ELISA. H high, L low γ-secretase activity. N = 3 independent experiments, one-sample t-test, *p < 0.05.
