Fig. 1: GABPA inhibits collagen formation and P4HA2 expression in mice. | Cell Death & Disease

Fig. 1: GABPA inhibits collagen formation and P4HA2 expression in mice.

From: The ETS transcription factor GABPA inhibits bladder cancer aggressiveness by repressing extracellular matrix deposition and mechanotransduction signaling

Fig. 1

A Dermis from wild-type (WT) and GABPA-transgenic C57BL/6 mice (GABPA-T) was stained with H & E (left) and Picrosirius Red (PSR) (right), respectively. Representatives are shown. B Polarization analysis of PSR staining using a NIKON Eclipse ci upright microscope. Under a polarized microscope, Col I is thick fibers orange-yellow or bright red fibers, whereas Col III is thin, green fibers (Top). Representatives are shown. The quantification of Col I and III in dermis from WT and GABPA-T mice was expressed as area ratio (%) (Bottom). Each group included 3 mice, and two slides from each mouse were analyzed. C and D P4HA2 downregulation mediated by GABPA. Skin tissues from 3 WT and 3 GABPA-T mice were analyzed for P4HA2 protein (C) and mRNA (D) levels using immunoblotting and qRT-PCR, respectively. * Indicates P < 0.05.

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