Fig. 7: GABPA-upregulated miR-30e expression targets P4HA2 in BC cells. | Cell Death & Disease

Fig. 7: GABPA-upregulated miR-30e expression targets P4HA2 in BC cells.

From: The ETS transcription factor GABPA inhibits bladder cancer aggressiveness by repressing extracellular matrix deposition and mechanotransduction signaling

Fig. 7

A miR-30e is among the top-expressed miRNAs in GABPA-high BC tumors. The TCGA BLCA tumors were divided into GABPA-high and low groups using a median value as the cutoff, and differentially expressed miRNAs were then identified between the two groups. B miR-30e levels are anti-correlated with P4HA2 expression in the TCGA BLCA tumors. C GABPA overexpression upregulates Dicer1 and miR-30e expression in J82 cells. Left: Immunoblotting and right: qRT-PCR analyses. D GABPA depletion downregulates Dicer1 and miR-30e expression in J82 and HT1197 cells. Left: Immunoblotting, middle and right: qRT-PCR analyses. E miR-30e mimics (miR-30e m) inhibit P4HA2 expression, Col I formation, and proliferation in J82 cells. Control and GABPA-depleted J82 cells were transfected with miR-30e m and then examined for P4HA2 and Col I level (left panel), and proliferation (middle and right panel). F miR-30e inhibitor (miR-30e i) treatment induces P4HA2 expression, Col I formation (Left), enhanced proliferation (middle), and invasion (right) of J82 cells. *, **, and *** indicate P < 0.05, 0.01, and 0.001, respectively.

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