Fig. 4: Interaction analysis of MCM7 and SHCBP1.
From: MCM7 promotes liver fibrosis by transcriptionally regulating IL11 via the SHCBP1-RACGAP1-STAT3 axis
A Silver staining was used to visualize interacting proteins with MCM7, which were enriched using an MCM7 antibody. The arrowhead indicates the predicted size of MCM7. B Distinct protein bands from the gel were subjected to mass spectrometry, and the top ten interacting partners are shown. C The peptide map of the marked protein band identifies it as SHCBP1. D Endogenous interaction between MCM7 and SHCBP1 was detected by Co-IP in HepG2 cells. E Exogenous interaction between FLAG/HA-tagged MCM7 and FLAG/HA-tagged SHCBP1 was validated by Co-IP in HepG2 cells. F Immunofluorescence (IF) analysis showing the colocalization of MCM7 and SHCBP1 in primary hepatocytes (PHCs) (scale bar: 5 μm). G, H Schematic diagrams of MCM7 (G) and SHCBP1 (H) truncations (top), and Co-IP assays analyzing the interaction domains of MCM7 and SHCBP1 (bottom).
