Fig. 1: NAMPT-mediated NAD⁺ biosynthesis regulates T cell function in OC microenvironment.

A, B Dimensionality reduction, clustering, and cell annotation of single-cell data from OC (GSE217517). A A total of 28 cell clusters were identified, with each color representing a distinct cell cluster. B Discrimination between malignant and non-malignant cells, along with cell annotation. Six cell types were identified among the non-malignant cells, with each color denoting a specific cell type. C The NAD⁺ content in T cells was found to be abnormally reduced during co-culture with OC cells. D Marker genes are used for the subtype identification of T cells. E T cell subsets can be clustered into five distinct cell types: HSC, NK cells, Mesangial cells, CD8⁺ T-cells, CD4⁺ T-cells. F The expression of the NAD⁺ synthesis enzyme NAMPT is closely correlated with the expression of T cell activity genes. G NAMPT shows a positive correlation with T cell anti-tumor signaling. H Transcriptional level detection of NAMPT overexpression in T cells. I Overexpression of NAMPT increases the NAD⁺ levels within T cells. J NAMPT promotes the expression of T cell chemotaxis receptors. K, L NAMPT enhances the expression of T cell anti-tumor factors IFNγ, GZMB and TNFα. M, N NAMPT upregulates the expression of T cell infiltration and activation markers (CD3D, CD69, and GZMB), while FK866 can effectively suppress T cell reactivation.