Fig. 4: Downregulation of S100A10 versus S100A11 differently impact on tumoral initiation and growth, depending on the fatty liver context. | Cell Death & Disease

Fig. 4: Downregulation of S100A10 versus S100A11 differently impact on tumoral initiation and growth, depending on the fatty liver context.

From: Comparative analysis of S100A10 and S100A11 in MASLD and hepatic cancer development revealed a tumor suppressive role for S100A10

Fig. 4

A Experimental design of the diethylnitrosamine (DEN) protocol, including AAV8 injections, CT-scan measurements, and sacrifice. B CT-scan analysis of the tumor number per mouse in DEN mice injected with AAV8-shCTL (n = 12), AAV8-shS100A10 (n = 14), or AAV8-shS100A11 (n = 13) at 8, 9, 10, and 11 months of age. C Tumor counting in the liver ex vivo, following dissection of the liver from DEN mice injected with AAV8-shCTL (n = 14), AAV8-shS100A10 (n = 13), or AAV8-shS100A11 (n = 13) at 11 months of age. D Tumor volume measured using CT-scan analysis in DEN mice injected with AAV8-shCTL (n = 346), AAV8-shS100A10 (n = 425), or AAV8-shS100A11 (n = 486) at 11 months of age. E Qualitative histopathological characterization of hepatocyte lesions (foci of cellular alteration, Adenoma or HCC) in DEN mice injected with AAV8-shCTL (n = 11), AAV8-shS100A10 (n = 14) or AAV8-shS100A11 (n = 11). F Experimental design of the HFD-DEN protocol, including AAV8 injections, feeding period with High Fat Diet and euthanasia. G MRI analysis of the tumor number per mouse in HFD-DEN mice injected with AAV8-shCTL (n = 12), AAV8-shS100A10 (n = 11–12), or AAV8-shS100A11 (n = 10–13) at 6, 7, and 8 months of age. H Tumor number per mouse at 8 months-old assessed by MRI analysis in HFD-DEN mice injected with AAV8-shCTL (n = 12), AAV8-shS100A10 (n = 11), or AAV8-shS100A11 (n = 10). I Tumor counting in the liver ex vivo, following dissection of liver from HFD-DEN mice injected with AAV8-shCTL (n = 12), AAV8-shS100A10 (n = 10), or AAV8-shS100A11 (n = 10), at 8 months of age. J Tumor volume measured using CT-scan analysis in DEN mice injected with AAV8-shCTL (n = 353), AAV8-shS100A10 (n = 664), or AAV8-shS100A11 (n = 230), at 11 months of age. Results are presented as means +/− S.E.M. “n” represents the number of animals or the number of tumors (D and J). * = p < 0.05; **** = p < 0.0001 determined by Two-Way ANOVA followed by Dunnett’s post-hoc analysis (B and G), by One-Way ANOVA followed by Dunnett’s post-hoc analysis (H, I), or by Kruskall–Wallis test followed by Dunnett’s post-hoc analysis (C, D, and J) or by t-test (Fig. 4G).

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