Fig. 4: Tumor microenvironment-mimicking co-culture induced resistance to BH3-mimetics in Diffuse Large B Cell Lymphoma (DLBCL) cell lines. | Cell Death & Disease

Fig. 4: Tumor microenvironment-mimicking co-culture induced resistance to BH3-mimetics in Diffuse Large B Cell Lymphoma (DLBCL) cell lines.

From: Systems biology-enabled targeting of NF-κΒ and BCL2 overcomes microenvironment-mediated BH3-mimetic resistance in DLBCL

Fig. 4

A Viability of RIVA cells cultured for 24 h with or without hCD40L-3T3 cells in the presence of 0.0001–100μΜ of the BCL-2 inhibitor ABT199 (venetoclax). Error bars representing the mean ± standard deviation of three independent experiments, normalized to the untreated control. B LC50 values for RIVA in each condition with error bars representing the mean ± standard deviation of three independent experiments, normalized to the untreated control (*P < 0.05, unpaired Ttest). C MCL1, BCL2, and BCLXL levels, shown as fold changes of 24-h stimulation with hCD40L-3T3 cells to unstimulated control in RIVA with error bars representing the mean ± standard deviation of five independent experiments (**P < 0.01, ***P < 0.001 one-way ANOVA with Tukey’s comparisons test. D Viability of SUDHL8 cells cultured for 24 h with or without hCD40L-3T3 cells in the presence of 0.0001–100 μΜ of the BCLXL inhibitor A1331852. Error bars representing the mean ± standard deviation of three independent experiments, normalized to the untreated control. E LC50 values for SUDHL8 in each condition with error bars representing the mean ± standard deviation of three independent experiments, normalized to the untreated control (***P < 0.001, unpaired Ttest). F MCL1, BCL2, and BCLXL levels, shown as fold changes of 24-h stimulation with hCD40L-3T3 cells to unstimulated control in SUDHL8 with error bars representing the mean ± standard deviation of five independent experiments (*P < 0.05, one-way ANOVA with Tukey’s comparisons test).

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