Fig. 5: Immunofluorescence microscopy reveals differential NF-κB basal RelA activity in Diffuse Large B-cell Lymphoma (DLBCL) cell lines.

A Schematic of the canonical and non-canonical NF-κB signaling pathway illustrating how non-canonical NF-κB activation results in the induction of BCLXL and how elevated basal canonical signaling induces multiple negative regulators of NF-κB which may contribute to cell line specific induction of MCL1. B Representative immunofluorescence microscopy following staining in RIVA cells for RelA (NF-κB), DAPI (nucleus), actin (cytoplasm), to measure nuclear and cytoplasmic subcellular localization. C Quantification immunofluorescence microscopy showing nuclear to cytoplasmic ratio of cRel and RelA in single cells. Data shows a representative replicate in the indicated cell lines. Violin width indicates data density. D Quantification of the nuclear to cytoplasmic ratio of cRel (top) and RelA (bottom) as bar graphs with error bars of the mean ± standard deviation of three independent experiments in RIVA, U2932 and SUDHL8 (*P < 0.05; One-Way ANOVA).