Fig. 4: Caspase-3 facilitates melanoma metastasis in vivo. | Cell Death & Disease

Fig. 4: Caspase-3 facilitates melanoma metastasis in vivo.

From: Atypical contribution of caspase-3 to melanoma cancer cell motility by regulation of coronin 1B activity

Fig. 4

A Workflow for the generation of primary zebrafish melanomas upon microinjection of the transposon-based MiniCoopR vector into one-cell stage embryos. B Western blot analysis showing the expression levels of Caspase-3 (CASP3) and Green Fluorescent Protein (GFP/Ctl) in primary zebrafish melanoma tumors. C Tumor-free survival curves of Tg (mitfa:BRAFV600E), tp53−/−, mitfa−/− zebrafish injected with vectors for the overexpression of CASP3 or GFP (Ctl). Statistical analysis was performed using the log-rank test. D Workflow for the transplantation of primary zebrafish melanomas into the dorsal cavity of secondary recipient Casper fish. E Representative images of Casper fish transplanted with primary zebrafish melanomas overexpressing CASP3 or GFP. Arrows indicate the location of disseminated melanoma cells. F Quantification of the percentage of secondary recipients with tumor spreading, comparing control GFP (n = 6) and CASP3 (n = 7) overexpressing tumors. Data are presented as mean ± standard deviation (S.D.); statistical analysis was performed using two-tailed t-test.

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