Table 1 Primers used in this study.

From: Atypical contribution of caspase-3 to melanoma cancer cell motility by regulation of coronin 1B activity

Table 1

Primer Forward (5’ → 3’)

Primer Reverse (5’ → 3’)

CRISPR-Cas9 crRNA

hCASP3

rGrGrArArGrCrGrArArUrCrArArUrGrGrArCrUrCrGrUrUrUrUrArGrArGrCrUrArUrGrCrU

/

Primers for qRT-PCR

hGAPDH

TGCACCACCAACTGCTTAGC

GGCATGGACTGTGGTCATGAG

hCASP3

CTCTGGTTTTCGGTGGGTGT

CTTCCATGTATGATCTTTGGTTCC

gRNA for lentiCRISPR V2

hCASP3_exon 1

CACCGGAGAACACTGAAAACTCAG

AAACCTGAGTTTTCAGTGTTCTCC

hCASP3_exon 2.1

CACCGGAAGCGAATCAATGGACTC

AAACGAGTCCATTGATTCGCTTCC

hCASP3_exon2.2

CACCGCATACATGGAAGCGAATCAA

AAACTTGATTCGCTTCCATGTATGC

BioID2 and GFP plasmid construct

pEGFP-N3-CASP3

GCGCCTCGAGCTATGGAGAACACTGAAAACTC

CGCGTCGACGTGATAAAAATAGAGTTCTTTTGTGAG

pcDNA3-mycBioID2-CASP3 (PCR n°1 for mycBioID2)

CTTGGTACCGAGCTCGGATCCGCCACCATGGAACAAAAACTCATCTCAG

CGCTGCCGCCGCTGCCGCCGCTTCTTCTCAGGCTGAAC

pcDNA3-mycBioID2-CASP3 (PCR n°2 for CASP3)

CGGCGGCAGCGGCGGCAGCGAGAACACTGAAAACTCAGTG

TGATGGATATCTGCAGAATTCTTAGTGATAAAAATAGAGTTCTTTTG

pcDNA3- CASP3-mycBioID2 (PCR n°1 for CASP3)

CTTGGTACCGAGCTCGGATCCGCCACCATGGAGAACACTGAAAAC

CGCTGCCGCCGCTGCCGCCGTGATAAAAATAGAGTTCTTTTGTG

pcDNA3- CASP3-mycBioID2 (PCR n°2 for mycBioID2)

CGGCGGCAGCGGCGGCAGCGAACAAAAACTCATCTCAGAAG

TGATGGATATCTGCAGAATTCTTAGCTTCTTCTCAGGCTGAACTC

pITR2-mycBioID2

CGCGGCCTCTGAGGCCGCCACCATGGAACAAAAACTC

CGCGGCCTGTCAGGCCTTAGCTTCTTCTCAGGCTGAAC

pITR2-mycBioID2-CASP3

CGCGGCCTCTGAGGCCGCCACCATGGAACAAAAACTC

CGCGGCCTGTCAGGCCTTAGTGATAAAAATAGAGTTCTTTTGTGAG

pITR2-CASP3-mycBioID2

CGCGGCCTCTGAGGCCGCCACCATGGAGAACACTGAAAAC

CGCGGCCTGTCAGGCCTTAGCTTCTTCTCAGGCTGAAC

TetON-XIAP

pSB_TET_XIAP_Oligo1

ACCCTCGAAAGGCCTCTGAGGCCATGACTTTTAACAGTTTTGAAGG

 

pSB_TET_XIAP_Oligo2

ATGGAAGCTTGGCCTGACAGGCCTTAAGACATAAAAATTTTTTGCTTG

 
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