Fig. 3: MRPL13 improves mitochondrial function in OC cells.

A, B Bubble plots of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results for the co-expressed genes of MRPL13 based on David database. C Results of GSEA comparing the enrichment of the oxidative phosphorylation pathway between patients with high MRPL13 expression and low MRPL13 expression in TCGA-OV cohort. D OCR was measured in OVCAR-3 and ES-2 cells with MRPL13 knockdown using Seahorse XF96 Extracellular Flux Analyzer. Basal respiration, maximal respiration and ATP production related OCR were quantitatively calculated. E Relative ATP level was recorded in OVCAR-3 and ES-2 cells with MRPL13 knockdown by ATP assay kit. F The mean fluorescence intensity (MFI) of DCF-DA was quantitatively calculated by flow cytometry to assess the ROS level in OVCAR-3 and ES-2 cells with MRPL13 knockdown. G Mitochondrial membrane potential in OVCAR-3 and ES-2 cells with MRPL13 knockdown was determined by quantitative analysis of the MFI of JC-1 aggregates (red)/monomers (green). H The degree of mPTP opening in OVCAR-3 and ES-2 cells with MRPL13 knockdown was detected by quantitative analysis of the MFI of Calcein AM fluorescent probe. I Fluorescence staining (Mito-Tracker Red CMXRos, red; Hoechst, blue) showing the mitochondrial morphology in OVCAR-3 and ES-2 cells with MRPL13 knockdown. The mitochondrial length was quantitatively analyzed by ImageJ Mitochondria Analyzer. J Representative images of TEM showing the mitochondrial morphology in OVCAR-3 and ES-2 cells with MRPL13 knockdown. All assays were performed in three independent experiments. Data are presented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.