Fig. 5: H19 expression is controlled by an HDAC1-mediated WT1/Sp1 switch. | Cell Death & Disease

Fig. 5: H19 expression is controlled by an HDAC1-mediated WT1/Sp1 switch.

From: Long noncoding RNA H19 promotes the acquisition of a mesenchymal-like invasive phenotype in mesothelial primary cells through an HDAC1-mediated WT1/Sp1 switch

Fig. 5

A Predicted WT1 (left) and Sp1 (middle) binding site on segment n3 on H19 promoter by PROMO. The overlapping sequence is shown in yellow (right). B RT-qPCR showing the expression of H19, from MeT5A cells upon WT1 genetic silencing (B left) WT1 ectopic expression (B middle) and Sp1 genetic silencing (C). L34 mRNA levels were used for normalization. Bars represent means ± SEM of 4 experiments. C Study of H3K27 acetylation of H19 promoter from ENCODE. WT1/Sp1 binding sites are marked in yellow. D Magnetic ChIP experiment showing H3 acetylation on H19 promoter (binding segment n3) using anti-H3Ac IgG and as control, with normal rabbit IgG on chromatin from MeT5A cells treated with DMSO (CTR) or with MS-275. Bars represent means ± SEM of 4 experiments. E, F ChIP experiment showing WT1 binding (E) and Sp1 binding (F) on H19 promoter upon treatment of MeT5A cells with MS-275 (250 nM) for 48 h and mutual genetic silencing. Bars represent means ± SEM of 4 experiments.

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