Table 2 qRT-PCR primers used in this study.

From: Long noncoding RNA H19 promotes the acquisition of a mesenchymal-like invasive phenotype in mesothelial primary cells through an HDAC1-mediated WT1/Sp1 switch

Name

Sequences

L34

F 5′-GTCCCGAACCCCTGGTAATAG-3′

R 5′-GGCCCTGCTGACATGTTTCTT-3′

TGFβRI

F 5′-AACTTCCAACTACTGGCCCT-3′

R 5′-GGTGAATGACAGTGCGGTTG-3′

TGFβ1

F 5′-AAGGACCTCGGCTGGAAGGTG-3′

R 5′-CCCGGGTTATGCTGGTTGTA-3′

SMAD2

F 5′-ACAGCTAGGCAGGGCAACTA-3′

R 5′-GGGCAGAGTTCACAGTCACA-3′

SMAD3

F 5′-CCCCAGAGCAATATTCCAGA-3′

R 5′-GACATCGGATTCGGGGATAG-3′

PAI-1

F 5′-AGTGGACTTTTCAGAGGTGGA-3′

R 5′-GCCGTTGAAGTAGAGGGCATT-3′

E-CADHERIN

F 5′-TACGCCTGGGACTCCACCTA-3′

R 5′-CCAGAAACGGAGGCCTGAT-3′

OCCLUDIN

F 5′-AAGGTCAAAGAGAACAGAGCAAGA-3′

R 5′-TATTCCCTGATCCAGTCCTCCTC-3′

HDAC1

F 5′-CATCGCTGTGAATTGGGCTG-3′

R 5′-CCCTCTGGTGATACTTTAGCAGT-3′

COL1A1

F 5′-AGCCAGCAGATCGAGAACAT-3′

R 5′-TCTTGTCCTTGGGGTTCTTG-3′

FN1

F 5′-GGCTGACAGAGAAGATTCCCG-3′

R 5′-AGCTGGGTCTGCTAACATCAC-3′

MMP14

F 5′-TCTGGCGGGTGAGGAATA-3′

R 5′-CTCTCGTAGGCAGTGTTGA-3′

α-SMA (ACTA2)

F 5′-CAGCCAAGCACTGTCAGG-3′

R 5′-CCAGAGCCATTGTCACAC-3′

H19

(Variant 1-2-3)

F 5′-GCCTTTGAATCCGGACACAA-3′

R 5′-GCTGTTCCGATGGTGTCTTT-3′

H19

(Variant 1-2)

F 5′-GAGGATGGTGCAGGCAGG-3′

R 5′-TCCTGCTTGTCACGTCCAC-3′

H19

(Variant 3)

F 5′-AGTTGAAGCCAGGTCTCCAG-3′

R 5′-CTCTGTCCTCGCCGTCAC-3′

H19 Promoter segment n1

F 5′- AAGGTCCAGAGGGAGACCCT -3′

R 5′- ACGTTTCTGTGGGTGAACCC -3′

H19 Promoter segment n2

F 5′- GGGTTCACCCACAGAAACGT -3′

R 5′- CAACCGATTCTGCACCATC -3′

H19 Promoter segment n3

F 5′- GATGGTGCAGAATCGGTTG -3′

R 5′- TCAGACACGTAGCCCAATGT -3′

H19 Promoter segment n4

F 5′- TTCCCCTTCTGTCTCACCAC -3′

R 5′- TCCCATGAGCGTCCTATTCC -3′

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