Fig. 6: Inhibition of ARF1 Activity Leads to Starvation-Induced Reduction of LD-mitochondrion Interactions and Lipid Accumulation. | Cell Death & Disease

Fig. 6: Inhibition of ARF1 Activity Leads to Starvation-Induced Reduction of LD-mitochondrion Interactions and Lipid Accumulation.

From: AMPK regulates ARF1 localization to membrane contact sites to facilitate fatty acid transfer between lipid droplets and mitochondria

Fig. 6

A Schematic representation of mice starved with or without BFA injection treatment. B Comparative statistical analysis of muscle cross-sectional area in mice. C HE staining and Oil Red O staining of mouse muscle tissue sections. D Determination of triglyceride content in mouse muscle tissue. E WB detection of PLIN2 and ARF1 protein expression levels in muscle tissues of mice after starvation and BFA treatment. F Quantification of WB results for ARF1 and PLIN2, normalized to GAPDH in group E. G WB detection of PLIN2, GAPDH, and TOM20 protein expression levels in muscle tissue mitochondria after starvation and BFA treatment. H Quantification of WB results for PLIN2, normalized to TOM20 in group G. I Detection of mRNA expression levels of lipid metabolism-related genes in muscle tissues using qPCR after starvation or feeding treatment in mice. J Detection of mRNA expression levels of lipid metabolism-related genes in muscle tissues using qPCR after mice were treated with or without BFA under starvation conditions. K WB analysis of lipid metabolism-related proteins in skeletal muscle tissues from nc, starvation, and starvation+BFA groups. GAPDH was used as a loading control. L Quantification of protein levels normalized to GAPDH. B, D, F, H, I, J, L Data are presented as means ± SD of three biologically independent replicates, analyzed by one-way ANOVA followed by Turkey’s post-hoc test.*p < 0.05, **p < 0.01, ***p < 0.001.

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