Fig. 7: FGF15 protects mice against sepsis-induced multi-organ inflammation by suppressing Irf7-mediated BMDM activation. | Cell Death & Disease

Fig. 7: FGF15 protects mice against sepsis-induced multi-organ inflammation by suppressing Irf7-mediated BMDM activation.

From: FGF15/FGFR4 signaling suppresses M1 macrophage polarization and multi-organ inflammation in septic mice by inhibiting H3K18 lactylation-driven Irf7 expression through NF2-Hippo activation

Fig. 7

Macrophage-depleted mice were subjected to the following treatments: Sham, CLP, CLP + transplantation with BMDMs (CLP + BMDM), CLP + transplantation with LPS-stimulated BMDMs (CLP + BMDMLPS), CLP + transplantation with rFGF15-treated, LPS-stimulated BMDMs (CLP + BMDMLPS + rFGF15), CLP + transplantation with rFGF15-treated, LPS-stimulated BMDMs transfected with empty vector (CLP + BMDMLPS + rFGF15 + oe-NC), or CLP + transplantation with rFGF15-treated, LPS-stimulated BMDMs transfected with Irf7-expressing vector (CLP + BMDMLPS + rFGF15 + oe-Irf7). A Survival analysis. n = 10, *p < 0.05. B Bacterial loads in peripheral blood and peritoneal lavage fluid (PLF). n = 9, *p < 0.05. C HE-stained images of the heart, liver, intestine, lung, and kidney showing immune cell infiltration. D, E Detection of Th17 (CD4 + IL17A + ) and Treg cells (CD4 + CD25 + FOXP3 + ) in peripheral blood by flow cytometry. D Density plots. E Quantified Th17/Treg ratio. n = 6, *p < 0.05. F Detection of TNF-α, IFN-γ, IL-6, and IL-17A in peripheral blood by ELISA. n = 10, *p < 0.05.

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