Fig. 1: CAD interacts with STING.
From: CAD hijacks STING to impair antitumor immunity and radiotherapy efficacy of colorectal cancer
A Representative image of silver staining of proteins co-immunoprecipitated with CAD. Red arrows indicated as the bands of CAD and STING protein. B CAD associated proteins determined by mass-spectrometry analysis. C, D Immunoprecipitation and western blotting analyzing the interaction between exogenous (C) or endogenous (D) CAD and STING in the indicated cells. E, F Representative confocal fluorescence microscopy images (E) and colocalization analyses (F) of CAD and STING. Scale bars, 10 μm. Blue arrows indicate the colocalization sites. G GST pull-down assays analyzing the interaction between CAD and STING. H SPR assay analyzing the binding affinity of STING protein with CAD protein. I Schematic representation of human STING and CAD mutants (upper). Immunoprecipitation and western blotting analyses of the interaction between STING mutants and CAD mutants (lower). J Molecular docking analyzing the interface between CAD and STING. K Immunoprecipitation and western blotting analyses of the interaction between CAD and the STINGGDV mutant. L Immunoprecipitation and western blotting analyses of the interaction between STING and the CADRCQ mutant.
